Recombinant Human Cytohesin 2, His-tagged

• Cat.No.: CYTH2-7184H
• Product Overview: Recombinant human CYTH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 48.9 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-399aa
• Description: CYTH2, also known as Cytohesin-2 and ARNO, is an ARF-1 guanine nucleotide exchange factor (GEF). ARF (ADP ribosylation factor) proteins, a group within the RAS superfamily, are GTP-binding proteins central to the process of vesicle budding. This protein promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Also it promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 10% glycerol,1mM DTT.
• Molecular Mass: 48.9 kDa (422aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEDGVYE PPDLTPEERM ELENIRRRKQ ELLVEIQRLR EELSEAMSEV EGLEANEGSK TLQRNRKMAM GRKKFNMDPK KGIQFLVENE LLQNTPEEIA RFLYKGEGLN KTAIGDYLGE REELNLAVLH AFVDLHEFTD LNLVQALRQF LWSFRLPGEA QKIDRMMEAF AQRYCLCNPG VFQSTDTCYV LSFAVIMLNT SLHNPNVRDK PGLERFVAMN RGINEGGDLP EELLRNLYDS IRNEPFKIPE DDGNDLTHTF FNPDREGWLL KLGGRVKTWK RRWFILTDNC LYYFEYTTDK EPRGIIPLEN LSIREVDDPR KPNCFELYIP NNKGQLIKAC KTEADGRVVE GNHMVYRISA PTQEEKDEWI KSIQAAVSVD PFYEMLAARK KRISVKKKQE QP
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CYTH2 cytohesin 2 [ Homo sapiens ]
• Official Symbol: CYTH2
• Synonyms: CYTH2; cytohesin 2; pleckstrin homology, Sec7 and coiled coil domains 2 , pleckstrin homology, Sec7 and coiled/coil domains 2 (cytohesin 2) , PSCD2, PSCD2L; cytohesin-2; ARNO; CTS18.1; Sec7p L; Sec7p like; ARF exchange factor; ARF nucleotide-binding site opener; PH, SEC7 and coiled-coil domain-containing protein 2; pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2); pleckstrin homology, Sec7 and coiled/coil domains 2 (cytohesin-2); CTS18; PSCD2; SEC7L; PSCD2L; Sec7p-L; Sec7p-like
• Gene ID: 9266
• mRNA Refseq: NM_004228
• Protein Refseq: NP_004219
• MIM: 602488
• UniProt ID: Q99418
• Chromosome Location: 19q13.32
• Pathway: Arf1 pathway, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem
• Function: ARF guanyl-nucleotide exchange factor activity; protein binding