Recombinant Human CYB5A Protein, His tagged

• Cat.No.: CYB5A-30H
• Product Overview: This recombinant human cytochrome b5 protein with Histidine tag was expressed in E. coli and is enzymatically active.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.
• Purity: ≥ 75% by visual inspection of 5 μg in a Coomassie stained SDS gel.
• Storage: Store at -70 centigrade. Avoid Multiple Freeze-Thaw Cycles.
• Storage Buffer: 10 mM Na/K phosphate, pH 7.25, 0.1 mM EDTA, 0.1 mM DTT, 0.01% CHAPS
• Concentration: 1.77 mg/mL total protein using the BCA protein assay with BSA as a standard.
• Reference: 1. Holmans et al. (1994) Arch. Biochem. Biophys. 312: 554-565.

Gene Information

• Gene Name: CYB5A cytochrome b5 type A (microsomal) [ Homo sapiens (human) ]
• Official Symbol: CYB5A
• Synonyms: CYB5A; cytochrome b5 type A (microsomal); CYB5, cytochrome b 5, cytochrome b5 (microsomal); cytochrome b5; type 1 cyt-b5; CYB5; MCB5
• Gene ID: 1528
• mRNA Refseq: NM_001190807
• Protein Refseq: NP_001177736
• MIM: 613218
• UniProt ID: P00167