Recombinant Human CUL2 Protein, His&GST-tagged

• Cat.No.: CUL2-1295H
• Product Overview: Recombinant Human CUL2 Protein (Val511-Ala745) with N-His&GST tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&GST
• ProteinLength: Val511-Ala745
• Description: Enables ubiquitin protein ligase binding activity. Predicted to be involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Predicted to act upstream of or within protein catabolic process. Located in nucleoplasm. Part of Cul2-RING ubiquitin ligase complex.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 57.1 kDa
  Accurate Molecular Mass: 61 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CUL2 cullin 2 [ Homo sapiens (human) ]
• Official Symbol: CUL2
• Synonyms: CUL2; cullin 2; cullin-2; CUL-2; MGC131970
• Gene ID: 8453
• mRNA Refseq: NM_001198777
• Protein Refseq: NP_001185706
• MIM: 603135
• UniProt ID: Q13617