Recombinant Human CRYGD, His-tagged

• Cat.No.: CRYGD-26245TH
• Product Overview: Recombinant full length Human CRYGD with an N terminal His tag; 194 amino acids with the tag; predicted MWt 22.9 kDa.

Specification

• Description: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation.
• Protein length: 174 amino acids
• Conjugation: HIS
• Molecular Weight: 22.900kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.08% DTT, 10% Glycerol, 1.17% Sodium chloride, 88% Tris buffer
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMGKITLYEDRGFQGRHYECSSDHPNLQPYLSRCNSARVDSGCWMLYEQPNYSGLQYFLRRGDYADHQQWMGLSDSVRSCRLIPHSGSHRIRLYEREDYRGQMIEFTEDCSCLQDRFRFNEIHSLNVLEGSWVLYELSNYRGRQYLLMPGDYRRYQDWGATNARVGSLRRVIDFS
• Sequence Similarities: Belongs to the beta/gamma-crystallin family.Contains 4 beta/gamma crystallin Greek key domains.

Gene Information

• Gene Name: CRYGD crystallin, gamma D [ Homo sapiens ]
• Official Symbol: CRYGD
• Synonyms: CRYGD; crystallin, gamma D; CRYG4; gamma-crystallin D
• Gene ID: 1421
• mRNA Refseq: NM_006891
• Protein Refseq: NP_008822
• Uniprot ID: P07320
• Chromosome Location: 2q33-q35
• Function: protein binding; structural constituent of eye lens