Recombinant Human CRYAB Protein, Myc/DDK-tagged

• Cat.No.: CRYAB-01H
• Product Overview: Recombinant protein of human crystallin, alpha B (CRYAB) with a C-Myc/DDK tag was expressed in HEK293T.

Specification

• Description: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants.
• Source: HEK293T
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 20 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: CRYAB crystallin alpha B [ Homo sapiens (human) ]
• Official Symbol: CRYAB
• Synonyms: CRYAB; crystallin alpha B; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101; alpha-crystallin B chain; epididymis secretory protein Li 101; heat shock protein beta-5; heat-shock 20 kD like-protein; renal carcinoma antigen NY-REN-27; rosenthal fiber component
• Gene ID: 1410
• mRNA Refseq: NM_001885
• Protein Refseq: NP_001876
• MIM: 123590
• UniProt ID: P02511