Recombinant Human CRYAB Protein, C-His-tagged

• Cat.No.: CRYAB-22H
• Product Overview: Recombinant Human CRYAB Protein, fused to His-tag, was expressed in E. coli.

Specification

• Description: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 21.2 kDa
• Protein length: 183aa
• AA Sequence: MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK
• N-terminal Sequence Analysis: 1-175aa
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Usage: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay).

Gene Information

• Gene Name: CRYAB crystallin alpha B [ Homo sapiens (human) ]
• Official Symbol: CRYAB
• Synonyms: MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
• Gene ID: 1410
• mRNA Refseq: NM_001885.3
• Protein Refseq: NP_001876.1
• MIM: 123590
• UniProt ID: P02511