Recombinant Human CRKL, His-tagged

• Cat.No.: CRKL-26624TH
• Product Overview: Recombinant full length Human CrkL with an N terminal His tag; 323 amino acids with tag, predicted MWt 35.9 kDa.

Specification

• Description: This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
• Protein length: 303 amino acids
• Conjugation: HIS
• Molecular Weight: 35.900kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 10% Glycerol, 0.03% DTT, 0.58% Sodium chloride
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMSSARFDSSDRSAWYMGPVS RQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSH YIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIE PAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAE DLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEK LVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVS GSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEV GDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPD ENE
• Sequence Similarities: Belongs to the CRK family.Contains 1 SH2 domain.Contains 2 SH3 domains.

Gene Information

• Gene Name: CRKL v-crk sarcoma virus CT10 oncogene homolog (avian)-like [ Homo sapiens ]
• Official Symbol: CRKL
• Synonyms: CRKL; v-crk sarcoma virus CT10 oncogene homolog (avian)-like; v crk avian sarcoma virus CT10 oncogene homolog like; crk-like protein
• Gene ID: 1399
• mRNA Refseq: NM_005207
• Protein Refseq: NP_005198
• MIM: 602007
• Uniprot ID: P46109
• Chromosome Location: 22q11.21
• Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem
• Function: SH3/SH2 adaptor activity; protein binding; protein tyrosine kinase activity; signal transducer activity