Recombinant Full Length Human F2 Protein, GST-tagged

• Cat.No.: F2-4495HF
• Product Overview: Human F2 full-length ORF ( NP_000497.1, 1 a.a. - 622 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 96.4 kDa
• Protein length: 622 amino acids
• AA Sequence: MAHVRGLQLPGCLALAALCSLVHSQ HVFLAPQQARSLLQRVRRANTFLEE VRKGNLERECVEETCSYEEAFEALE SSTATDVFWAKYTACETARTPRDKL AACLEGNCAEGLGTNYRGHVNITRS GIECQLWRSRYPHKPEINSTTHPGA DLQENFCRNPDSSTTGPWCYTTDPT VRRQECSIPVCGQDQVTVAMTPRSE GSSVNLSPPLEQCVPDRGQQYQGRL AVTTHGLPCLAWASAQAKALSKHQD FNSAVQLVENFCRNPDGDEEGVWCY VAGKPGDFGYCDLNYCEEAVEEETG DGLDEDSDRAIEGRTATSEYQTFFN PRTFGSGEADCGLRPLFEKKSLEDK TERELLESYIDGRIVEGSDAEIGMS PWQVMLFRKSPQELLCGASLISDRW VLTAAHCLLYPPWDKNFTENDLLVR IGKHSRTRYERNIEKISMLEKIYIH PRYNWRENLDRDIALMKLKKPVAFS DYIHPVCLPDRETAASLLQAGYKGR VTGWGNLKETWTANVGKGQPSVLQV VNLPIVERPVCKDSTRIRITDNMFC AGYKPDEGKRGDACEGDSGGPFVMK SPFNNRWYQMGIVSWGEGCDRDGKY GFYTHVFRLKKWIQKVIDQFGE
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: F2 coagulation factor II (thrombin) [ Homo sapiens ]
• Official Symbol: F2
• Synonyms: F2; coagulation factor II (thrombin); prothrombin; serine protease; prothrombin B-chain; PT; THPH1
• Gene ID: 2147
• mRNA Refseq: NM_000506
• Protein Refseq: NP_000497
• MIM: 176930
• UniProt ID: P00734

Reviews

01/01/2022

Quality control is evident. This product ensures accuracy in our studies.

01/27/2019

Our lab swears by this protein. Fast shipping is a definite bonus.

12/18/2018

Consistently reliable. The shipping speed is appreciated in our fast-paced lab.

Q&As

Can you discuss the role of the F2 gene in the synthesis of vitamin K-dependent clotting factors, and how disruptions in this process may lead to coagulation disorders?

The F2 gene plays a crucial role in the synthesis of vitamin K-dependent clotting factors, including prothrombin. Disruptions in this process, often due to genetic or nutritional factors, can contribute to coagulation disorders, impacting hemostasis and blood clotting.

How is the F2 gene regulated at the transcriptional level, and what factors influence prothrombin synthesis in hepatocytes?

Transcriptional regulation of the F2 gene involves factors like vitamin K-dependent gamma-carboxylation. Hepatocytes synthesize prothrombin under the influence of vitamin K and other coagulation factors.

The F2 gene encodes prothrombin, a crucial factor in blood coagulation. Prothrombin is converted to thrombin, a key enzyme in the clotting cascade, facilitating fibrin formation.

The F2 gene encodes prothrombin, a crucial factor in blood coagulation. Prothrombin is converted to thrombin, a key enzyme in the clotting cascade, facilitating fibrin formation.

What are the implications of genetic variations in the F2 gene, particularly polymorphisms, on prothrombin function and susceptibility to thrombotic or bleeding disorders?

Genetic variations in F2, including polymorphisms, can influence prothrombin function. Certain variants may predispose individuals to thrombotic or bleeding disorders.

How does genetic variation in the F2 gene impact prothrombin levels, and what are the consequences for an individual's risk of thrombotic events?

Genetic variations in the F2 gene can influence prothrombin levels, affecting an individual's propensity for thrombotic events. Certain variants may predispose individuals to a higher or lower risk of abnormal blood clot formation.