Recombinant Human CH25H, His-tagged

• Cat.No.: CH25H-11153H
• Product Overview: Recombinant Human CH25H protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 140-272a.a.
• Description: This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: CH25H cholesterol 25-hydroxylase [ Homo sapiens ]
• Official Symbol: CH25H
• Synonyms: CH25H; cholesterol 25-hydroxylase; h25OH; cholesterol 25-monooxygenase; C25H
• Gene ID: 9023
• mRNA Refseq: NM_003956
• Protein Refseq: NP_003947
• MIM: 604551
• UniProt ID: O95992
• Chromosome Location: 10q23
• Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Primary bile acid biosynthesis, organism-specific biosystem; Primary bile acid biosynthesis, conserved biosystem; Synthesis of bile acids and bile salts, organism-specific biosystem
• Function: cholesterol 25-hydroxylase activity; iron ion binding; metal ion binding; steroid hydroxylase activity