Recombinant Human CH25H Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CH25H-4169H
• Product Overview: CH25H MS Standard C13 and N15-labeled recombinant protein (NP_003947) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 31.7 kDa
• AA Sequence: MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPARTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CH25H cholesterol 25-hydroxylase [ Homo sapiens (human) ]
• Official Symbol: CH25H
• Synonyms: CH25H; cholesterol 25-hydroxylase; h25OH; cholesterol 25-monooxygenase; C25H
• Gene ID: 9023
• mRNA Refseq: NM_003956
• Protein Refseq: NP_003947
• MIM: 604551
• UniProt ID: O95992