Recombinant Human CH25H 293 Cell Lysate

• Cat.No.: CH25H-7549HCL

Specification

• Description: Antigen standard for cholesterol 25-hydroxylase (CH25H) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: CH25H cholesterol 25-hydroxylase [ Homo sapiens ]
• Official Symbol: CH25H
• Synonyms: CH25H; cholesterol 25-hydroxylase; h25OH; cholesterol 25-monooxygenase; C25H
• Gene ID: 9023
• mRNA Refseq: NM_003956
• Protein Refseq: NP_003947
• MIM: 604551
• UniProt ID: O95992
• Chromosome Location: 10q23
• Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Primary bile acid biosynthesis, organism-specific biosystem; Primary bile acid biosynthesis, conserved biosystem; Synthesis of bile acids and bile salts, organism-specific biosystem
• Function: cholesterol 25-hydroxylase activity; iron ion binding; metal ion binding; steroid hydroxylase activity