Recombinant Human Centromere Protein A, His-tagged

• Cat.No.: CENPA-617H
• Product Overview: Recombinant Human CENP-A produced inSF9is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENP-A is expressed with a -6x His tag and purified by proprietary chromatographic techniques

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-A is another important centromeric autoantigen in addition to CENP-B: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENP-A antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENP-B-autoantibody negative patients.
• Physical Appearance: Sterile Filtered liquid formulation.
• Formulation: CENP-A is supplied at a concentration of 0.6 mg/ml in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea.
• Immunological Functions: Binds IgG-type human auto-antibodies.Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
• Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Coating Concentration: 0.15-0.4 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: CENPA centromere protein A [ Homo sapiens ]
• Synonyms: centromere protein A; CENP-A; CENPA; centromere protein A, 17kDa; centromere protein A (17kD); OTTHUMP00000122589; Centromere autoantigen A
• Gene ID: 1058
• mRNA Refseq: NM_001042426
• Protein Refseq: NP_001035891
• MIM: 117139
• UniProt ID: P49450
• Chromosome Location: 2p24-p21
• Pathway: Cell Cycle, Mitotic
• Function: DNA binding; chromatin binding; protein binding