Recombinant Human CENPB Protein, MYC/DDK-tagged

• Cat.No.: CENPB-3184H
• Product Overview: Recombinant Human CENPB protein, fused to MYC/DDK-tagged at C-terminus, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 65 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: CENPB centromere protein B, 80kDa [ Homo sapiens ]
• Official Symbol: CENPB
• Synonyms: OTTHUMP00000030132; centromere autoantigen B; centromere protein B; centromere protein B (80kD); centromere protein B, 80kDa
• Gene ID: 1059
• mRNA Refseq: NM_001810
• Protein Refseq: NP_001801
• MIM: 117140
• UniProt ID: P07199