Recombinant Human CENPB, His-tagged

• Cat.No.: CENPB-27393TH
• Product Overview: Recombinant Full length Human CENPB produced in Sf9 insect cells with a 6x His tag, 65,940 Dalton.

Specification

• Species: Human
• Tag: His
• Protein Length: 1-599 aa
• Description: This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
• Conjugation: HIS
• Biological activity: CENPB-27393TH reacts with Scleroderma patient sera.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 250mM Sodium chloride, 20mM HEPES, pH 7.6
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Full Length: Full L.

Gene Information

• Gene Name: CENPB centromere protein B, 80kDa [ Homo sapiens ]
• Official Symbol: CENPB
• Synonyms: CENPB; centromere protein B, 80kDa; centromere protein B (80kD); major centromere autoantigen B
• Gene ID: 1059
• mRNA Refseq: NM_001810
• Protein Refseq: NP_001801
• MIM: 117140
• Uniprot ID: P07199
• Chromosome Location: 20p13
• Pathway: FOXM1 transcription factor network, organism-specific biosystem
• Function: centromeric DNA binding; chromatin binding; satellite DNA binding; sequence-specific DNA binding