Recombinant Human CDH1 Protein, His-tagged

• Cat.No.: CDH1-214H
• Product Overview: Biotinylated (In vitro) Recombinant Human Cadherin-1 is produced by Human 293 Cells. The target gene encoding Q23-A709 is expressed with an 8His tag at the C terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 23-709 a.a.
• Description: E-Cadherin is a calcium-dependent cell adhesion glycoprotein that belongs to the cadherin family. E-Cadherin is expressed in non-neural epithelial tissues. It tends to interact with each other in a homophilic manner in connecting cells. E-Cadherin clusters are critical components of adherent junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. E-Cadherin loss contributes to cancer metastasis. The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation.
• Form: Lyophilized frum a 0.2 um filtered solution of PBS, pH7.2.
• Molecular Mass: Predicted Molecular Weight: 79.6kDa
  Apparent Molecular Weight: Biotinylated Cadherin-1 appeared between 66.2-116kDa in a reducing SDS-PAGE gel.
• AA Sequence: Q23-A709
• Endotoxin: Less than 0.1 EU/ug.
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: greater than 95% as determined by reducing SDS-PAGE
• Shipping: The product is shipped on dry ice pack.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: CDH1 cadherin 1 [ Homo sapiens (human) ]
• Official Symbol: CDH1
• Synonyms: Cadherin-1; CAM120/80; E-cadherin; CD324; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1
• Gene ID: 999
• mRNA Refseq: NM_001317184.2
• Protein Refseq: NP_001304113.1
• MIM: 192090
• UniProt ID: P12830