Active Recombinant Mouse Cdh1 Protein

• Cat.No.: Cdh1-1719M
• Product Overview: Recombinant Mouse Cdh1 protein without tag, was expressed in NSO Cells.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Non
• Description: Cadherin 1, type 1, E-cadherin (epithelial), also known as CDH1 and CD32, is a tumor suppressor gene. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. E-cadherin-mediated cell-cell adhesion pathway may represent a novel chemotherapeutic target for bladder cancer, prostate cancer, and renal-cell carcinoma.
• Form: Lyophilized
• Bio-activity: The biological activity of Mouse E-CAD was determined by the ability of the immobilized protein to support the adhesion of the MCF7 human breast adenocarcinoma cells. When 5 x 10^4 cells/well are added to rmECAD/Fc Chimera coated plates (1.5 μg/ml with 100 μl/well), approximately 50-70% will adhere after 90 minutes at room temperature.
  Each investigator should determine their own optimal working dilution for specific applications.
• Molecular Mass: The predicted molecular weight of Recombinant Mouse ECAD is Mr 88.2 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 120 kDa.
• AA Sequence: dwvippiscpenekgefpknlvqiksnrdketkvfysitgqgadkppvgvfiieretgwlkvtqpldreaiakyilyshavssngeavedpmeivitvtdqndnrpeftqevfegsvaegavpgtsvmkvsatdadddvntynaaiaytivsqdpelphknmftvnrdtgvisvltsgldresyptytlvvqaadlqgeglsttakavitvkdindnapvfnpstyqgqvpenevnariatlkvtdddapntpawkavytvvndpdqqfvvvtdpttndgilktakgldfeakqqyilhvrveneepfegslvpstatvtvdvvdvneapifmpaerrvevpedfgvgqeitsytarepdtfmdqkityriwrdtanwleinpetgaiftraemdredaehvknstyvaliiatddgspiatgtgtlllvlldvndnapipeprnmqfcqrnpqphiitildpdlppntspftaelthgasvnwtieyndaaqeslilqprkdleigeykihlkladnqnkdqvttldvhvcdcegtvnncmkagivaaglqviegrmdpkscdkthtcppcpapellggpsvflfppkpkdtlmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwlngkeykckvsnkalpapiektiskakgqprepqvytlppsrdeltknqvsltclvkgfypsdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslslspgkhhhhhh
• Endotoxin: <1.0 EU/μg as determined by the LAL method
• Purity: >90% by SDS-PAGE and analyzed by silver stain.
• Notes: Products are for research use only. Not for use in diagnostic or therapeutic procedures.
• Storage: This lyophilized protein is stable for six to twelve months when stored desiccated at -20 centigrade to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 centigrade to 8 centigrade for one month or at -20 centigrade to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Storage Buffer: This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution containing 50mM Tris-Citrate, 50 mM NaCl and 2 mM CaCl2, pH 6.5.

Gene Information

• Gene Name: Cdh1
• Official Symbol: Cdh1 cadherin 1 [ Mus musculus (house mouse) ]
• Synonyms: Cdh1; cadherin 1; Um; UVO; Ecad; ARC-1; E-cad; L-CAM; AA960649; cadherin-1; E-cadherin; epithelial cadherin; uvomorulin
• Gene ID: 12550
• mRNA Refseq: NM_009864
• Protein Refseq: NP_033994