Recombinant Human CD8A protein, His-Avi-tagged, Biotinylated

Cat.No. : CD8A-6744H
Product Overview : Biotinylated Recombinant Human CD8A protein(P01732-1)(Ser22-Asp182), fused with C-terminal His and Avi tag, was expressed in HEK293.
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Species : Human
Source : HEK293
Tag : Avi&His
Protein Length : Ser22-Asp182
Tag : C-His-Avi
Conjugation/Label : Biotin
Form : Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Anti-CD8 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD8 alpha, His Tag with the EC50 of 4.8ng/ml determined by ELISA.
Molecular Mass : The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Tris-Bis PAGE result.
Endotoxin : Less than 1EU per μg by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Storage : Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution : Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Gene Name CD8A CD8a molecule [ Homo sapiens ]
Official Symbol CD8A
Synonyms CD8A; CD8a molecule; CD8, CD8 antigen, alpha polypeptide (p32) , T cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; p32; Leu2;
Gene ID 925
mRNA Refseq NM_001145873
Protein Refseq NP_001139345
MIM 186910
UniProt ID P01732

Not For Human Consumption!

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