Recombinant Human CD74, His-tagged

• Cat.No.: CD74-114H
• Product Overview: Recombinant human CD74, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: CD74, also known as HLA class II histocompatibility antigen gamma chain or HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74). The nascent MHC class II protein in the rough ER has its peptide-binding cleft blocked by the invariant chain (Ii; a trimer) to prevent it from binding cellular peptides or peptides from the endogenous pathway. The invariant chain also facilitates MHC class II"s export from the ER in a vesicle. The signal for endosomal targeting resides in the cytoplasmic tail of the invariant chain. This fuses with a lateendosome containing the endocytosed, degraded proteins. It is then cleaved by cathepsin S (cathepsin L in cortical thymic epithelial cells), leaving only a small fragment calle CLIP which blocks peptide binding until HLA-DM binds to MHC II, releasing CLIP and allowing other peptides to bind. The stable MHC class-II is then presented on the cell surface.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid. In phosphate buffered saline (pH7.4) containing 10% glycerol.
• Molecular Mass: 19.05kDa (166aa)18-28kDa (SDS-PAGE under reducing conditions.)
• AA Sequence: QQQGRLDKLT VTSQNLQLEN LRMKLPKPPK PVSKMRMATP LLMQALPMGA LPQGPMQNAT KYGNMTEDHV MHLLQNADPL KVYPPLKGSFPENLRHLKNT METIDWKVFE SWMHHWLLFE MSRHSLEQKP TDAPPKESLE LEDPSSGLGV TKQDLGPVPM HHHHHH
• Endotoxin: < 1.0 eu per 1 microgram of protein (determined by lal
• Purity: >85% by SDS - PAGE.
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by absorbance at 280nm)

Gene Information

• Gene Name: CD74 CD74 molecule, major histocompatibility complex, class II invariant chain [ Homo sapiens ]
• Official Symbol: CD74
• Synonyms: CD74; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen associated) , DHLAG; HLA class II histocompatibility antigen gamma chain; gamma chain of class II antigens; HLA DR gamma; Ia associated invariant chain; MHC HLA DR gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); II; DHLAG; HLADG; Ia-GAMMA; FLJ98970
• Gene ID: 972
• mRNA Refseq: NM_001025158
• Protein Refseq: NP_001020329
• MIM: 142790
• UniProt ID: P04233
• Chromosome Location: 5q31-q33
• Pathway: Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Tuberculosis, organism-specific biosystem; Tuberculosis, conserved biosystem
• Function: MHC class II protein binding; MHC class II protein binding; beta-amyloid binding; cytokine binding; cytokine receptor activity; identical protein binding; nitric-oxide synthase binding