Recombinant Human CCNC, His tagged

• Cat.No.: CCNC-08H
• Product Overview: The Recombinant Human Cyclin C (1-283aa), His-tagged, fused with 6* his tag, was expressed in E. coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-283 a.a.
• Description: CCNC (cyclin C) is a member of the cyclin family of proteins. This protein interacts with cyclin-dependent kinase 8 (CDK8) and induces the phosphorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this protein peaks in the G1 phase of the cell cycle. It has been shown that a non-cdk8-associated cellular pool of CCNC combines with cdk3 to stimulate pRb phosphorylation at S807/811 during the G0/G1 transition, and that this phosphorylation is required for cells to exit GO efficiently. Other results have shown that a marked elevated immunoreactivity of CCNC was found both in neurons and astrocytes in Alzheimer"s disease (AD). Increased levels of CCNC RNA were detected by ribonuclease protection assay (RPA) in severe AD cases and colocalization of CCNC and its preferred binding partner, CDK8, was only observed in astrocytes but not in neurons.
• AA sequence: 1-283aa
• Purification: Ni-sepharose
• Storage buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15% glycerol.
• Storage: The protein is stored in PBS buffer at -20°C.Repeated freeze/thaw cycles should be avoided.

Gene Information

• Gene Name: CCNC cyclin C[ Homo sapiens ]
• Official Symbol: CCNC
• Synonyms: CCNC; cyclin C; CycC; OTTHUMP00000016897; hSRB11; SRB11 homolog; cyclin-C
• Gene ID: 892
• mRNA Refseq: NM_001013399
• Protein Refseq: NP_001013417
• MIM: 123838
• UniProt ID: P24863
• Chromosome Location: 6q21
• Pathway: Gene Expression
• Function: protein binding