Recombinant Human CAST, GST-tagged

• Cat.No.: CAST-10743H
• Product Overview: Recombinant Human CAST protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: N-term-350a.a.
• Description: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: CAST calpastatin [ Homo sapiens ]
• Official Symbol: CAST
• Synonyms: CAST; calpastatin; calpain inhibitor; sperm BS-17 component; BS-17; MGC9402
• Gene ID: 831
• mRNA Refseq: NM_001042440
• Protein Refseq: NP_001035905
• MIM: 114090
• UniProt ID: P20810
• Chromosome Location: 5q14-q22
• Function: calcium-dependent cysteine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; protein binding