Recombinant Human CASP9 Protein, 1-416, C-His tagged

• Cat.No.: CASP9-35H
• Product Overview: Recombinant Human CASP9 Protein (1-416) with C-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 1-416(end)
• Description: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
• Form: Aqueous buffer solution
• Bio-activity: 0.11 pmol/min/μg
• Purity: ≥ 90%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.
• Notes: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Usage: 50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 0.1% Chaps, 5% glycerol, 10 mM DTT, and 10 μM Caspase 9 substrate. Incubate for 2 hours at room temperature. Fluorescence intensity is measured at exc380/em505 nm.
• Stability: At least 6 months at -80 centigrade.
• Storage: At -80 centigrade (dry ice)
• Storage Buffer: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, 65 mM imidazole
• References: 1. Guerrero AD., et al., J Clin Cell Immunol.9;3(3) (2012)
  2. Luo W., et al., Int J Biol Macromol. S0141-8130(13)00222-5. (2013)

Gene Information

• Gene Name: CASP9 caspase 9, apoptosis-related cysteine peptidase [ Homo sapiens (human) ]
• Official Symbol: CASP9
• Synonyms: CASP9; caspase 9, apoptosis-related cysteine peptidase; caspase 9, apoptosis related cysteine protease; caspase-9; APAF 3; ICE LAP6; MCH6; PPP1R56; protein phosphatase 1; regulatory subunit 56; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56; APAF3; APAF-3; ICE-LAP6; CASPASE-9c
• Gene ID: 842
• mRNA Refseq: NM_001229
• Protein Refseq: NP_001220
• MIM: 602234
• UniProt ID: P55211