Recombinant Human CASP9 protein, His-tagged

• Cat.No.: CASP9-627H
• Product Overview: Recombinant Human CASP9 aa. (Ala331~Leu411 (Accession # P55211)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala331~Leu411
• Description: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 10.7kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.1.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O

Gene Information

• Gene Name: CASP9 caspase 9 [ Homo sapiens (human) ]
• Official Symbol: CASP9
• Synonyms: CASP9; caspase 9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; caspase-9; ICE-like apoptotic protease 6; apoptotic protease MCH-6; apoptotic protease activating factor 3; caspase 9, apoptosis-related cysteine peptidase; protein phosphatase 1, regulatory subunit 56
• Gene ID: 842
• mRNA Refseq: NM_001229.4
• Protein Refseq: NP_001220.2
• UniProt ID: P55211