Recombinant Rat Casp9 protein, His-tagged

• Cat.No.: Casp9-629R
• Product Overview: Recombinant Rat Casp9 aa. (Met1~His200) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• ProteinLength: Met1~His200
• Description: This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
• Form: Freeze-dried powder
• Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 6.1.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Casp9 caspase 9 [ Mus musculus (house mouse) ]
• Official Symbol: Casp9
• Synonyms: Casp9; caspase 9; Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9; ICE-like apoptotic protease 6; apoptotic protease Mch-6; apoptotic protease-activating factor 3
• Gene ID: 12371
• mRNA Refseq: NM_001277932.1
• Protein Refseq: NP_001264861.1
• UniProt ID: A2AS93