Active Recombinant Human CASP3 Protein, His-tagged

• Cat.No.: CASP3-01H
• Product Overview: Human caspase 3 (CASP3), also known as CPP32 and apopain, a.a. 1 – 277 (end) with C-terminal His-tag, expressed in an E. coli expression system.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-277
• Description: The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
• Form: Aqueous buffer solution
• Bio-activity: ≥ 6,784 pmol/min/µg
• Molecular Mass: 17 kDa and 13 kDa
• AA Sequence: MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYHHHHHHH
• Purity: > 99%
• Applications: Enzyme kinetics, screening inhibitors, and selectivity profiling
• Stability: > 6 months
• Storage: Store at -80 centigrade
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT

Gene Information

• Gene Name: CASP3 caspase 3 [ Homo sapiens (human) ]
• Official Symbol: CASP3
• Synonyms: CASP3; caspase 3; CPP32; SCA-1; CPP32B; caspase-3; CASP-3; CPP-32; PARP cleavage protease; SREBP cleavage activity 1; apopain; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3; protein Yama
• Gene ID: 836
• mRNA Refseq: NM_004346
• Protein Refseq: NP_004337
• MIM: 600636
• UniProt ID: P42574