Recombinant Human Carbonic Anhydrase I, His-tagged

• Cat.No.: CA1-2505H
• Product Overview: Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 31.0 kDa.

Specification

• Description: CA1 is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O<->HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
• Molecular Mass: 31.0 kDa (281aa), confirmed by MALDI-TOF
• Protein length: 1-261aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGS LT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CA1 carbonic anhydrase I [ Homo sapiens ]
• Official Symbol: CA1
• Synonyms: CA1; carbonic anhydrase I; carbonic anhydrase 1; Car1; carbonic anhydrase B; carbonic dehydratase; carbonate dehydratase I; CAB; CA-I
• Gene ID: 759
• mRNA Refseq: NM_001128829
• Protein Refseq: NP_001122301
• MIM: 114800
• UniProt ID: P00915
• Chromosome Location: 8q21.2
• Pathway: C-MYB transcription factor network, organism-specific biosystem
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding