Recombinant Human Carbonic Anhydrase I, His-tagged

• Cat.No.: CA1-1677H
• Product Overview: Recombinant carbonic anhydrase fused to His-tag, was expressed in E. coli and purified by conventional chromatography techniques.

Specification

• Cat. No.: CA1-1677H
• Description: Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O HCO3 + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life.
• Concentration: 1 mg/ml
• Source: Human
• Host: E.coli
• Form: Supplied as a liquid in 20mM Tris, pH 8.0, containing 1mM DTT.
• Purity: > 95% by SDS - PAGE
• Sequence: 1-220 amino acids: MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDV
• Molecular Mass: 27 kDa (240aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: CA1 carbonic anhydrase I [ Homo sapiens ]
• Official Symbol: CA1
• Synonyms: CA1; carbonic anhydrase I; Car1; EC 4.2.1.1; Carbonic anhydrase 1; CA-I; Carbonic anhydrase B; CAB; carbonic dehydratase
• Gene ID: 759
• mRNA Refseq: NM_001128829
• Protein Refseq: NP_001122301
• MIM: 114800
• UniProt ID: P00915
• Chromosome Location: 8q21.2
• Pathway: Nitrogen metabolism
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding; carbonic dehydratase