Recombinant Human BRICK1, SCAR/WAVE actin-nucleating complex subunit, His-tagged

• Cat.No.: BRK1-416H
• Product Overview: BRK1, 1-75aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-75 a.a.
• Description: BRK1 involved in regulation of actin and microtubule organization. This protein is a part of a WAVE complex that activates the Arp2/3 complex. It belongs to the BRK1 family. Recombinant human BRK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.
• Molecular Mass: 11 kDa (98aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGQEDP VQREIHQDWA NREYIEIITS SIKKIADFLN SFDMSCRSRL ATLNEKLTAL ERRIEYIEAR VTKGETLT
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: BRK1 BRICK1, SCAR/WAVE actin-nucleating complex subunit [ Homo sapiens ]
• Official Symbol: BRK1
• Synonyms: BRK1; BRICK1, SCAR/WAVE actin-nucleating complex subunit; C3orf10, chromosome 3 open reading frame 10; protein BRICK1; BRICK1; SCAR/WAVE actin nucleating complex subunit; homolog (Arabidopsis thaliana); haematopoietic stem/progenitor cell protein 300; HSPC300; MDS027; probable protein BRICK1; BRICK1, SCAR/WAVE actin-nucleating complex subunit, homolog; hHBrk1; C3orf10
• Gene ID: 55845
• mRNA Refseq: NM_018462
• Protein Refseq: NP_060932
• MIM: 611183
• UniProt ID: Q8WUW1
• Chromosome Location: 3p25.3
• Pathway: ErbB1 downstream signaling, organism-specific biosystem; RAC1 signaling pathway, organism-specific biosystem; Regulation of Actin Cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, conserved biosystem