Recombinant Human BMP8B, His-tagged

• Cat.No.: BMP8B-18H
• Product Overview: Recombinant Human BMP8B encoding the human bone morphogenetic protein-8B (Leu25-Phe349) was recombined with Hexa-histidine tag at the N-Terminus. That was expressed inE.Coli,and purified by Ni-NTA column. MW = 35.8KD.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 25-349 a.a.
• Description: Bone morphogenetic protein 8B is a protein that in humans is encoded by the BMP8B gene. The protein encoded by this gene is a member of the TGF-β superfamily. It has close sequence homology to BMP7 and BMP5 and is believed to play a role in bone and cartilage development. It has been shown to be expressed in the hippocampus of murine embryos. The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth.
• Amino Acid Sequence: human bone morphogenetic protein 8B (Leu25-Phe349). 25LRPPPGCPQR RLGARERRDV QREILAVLGL PGRPRPRAPPAASRLPASAP LFMLDLYHAM AGD DDEDGAP AERRLGRADLVMSFVNMVER DRALGHQEPH WKEFRFDLTQ IPA GE AVTAAEFRIYKVPSI HLLNRTLHVS MFQVVQEQSN RESD LFF LDL QTL RAG DEGW LVLDVTAASD CWLLKRHKDL GLRL YVETED GETWT GWGWT KDS RLQ MWKL RLSRTPWVLS LHAPGPAQAPAERPLLCLLQ GHCPAS PSPI RT PRAVRPLR RRQPKKSNELPQANRLPGIF DDVRGSHGRQ VCRR HELYVS DSPCF 349.
• Application: Cell biology.
• Purity: >95% in SDS gel.
• Formulation: Lyophilized 100 μg human bone morphogenetic protein-8B in 50 μl of TBS ( 20 mM Tris, 50mM NaCl,pH8.0 ). Carry free.
• Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200 μg/mL . Allow to set at least 30 minutes at 4° C, mix well.
• Storage: Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein is stable for up to 6 months from date of receipt at - 20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
• Pathways: Cytokine-cytokine receptor interaction; Hedgehog signaling pathway; TGF-beta signaling pathway

Gene Information

• Gene Name: BMP8B bone morphogenetic protein 8b [ Homo sapiens ]
• Synonyms: bone morphogenetic protein 8b; OP2; BMP8; MGC131757; BMP8B; osteogenic protein 2; bone morphogenetic protein 8 (osteogenic protein 2); dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2)); dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2)); OP-2l; BMP-8; BMP-8B; OTTHUMP00000010836; Osteogenic protein 2; bone morphogenetic protein 8b
• Gene ID: 656
• mRNA Refseq: NM_001720
• Protein Refseq: NP_001711
• MIM: 602284
• UniProt ID: P34820
• Chromosome Location: 1p35-p32
• Function: cytokine activity; growth factor activity protein binding