Recombinant Human BMP8B 293 Cell Lysate

Cat.No. : BMP8B-8428HCL
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Description : Antigen standard for bone morphogenetic protein 8b (BMP8B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name BMP8B bone morphogenetic protein 8b [ Homo sapiens ]
Official Symbol BMP8B
Synonyms BMP8B; bone morphogenetic protein 8b; BMP8, bone morphogenetic protein 8 (osteogenic protein 2); bone morphogenetic protein 8B; OP 2; osteogenic protein 2; BMP-8; BMP-8B; bone morphogenetic protein 8 (osteogenic protein 2); dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2)); OP2; BMP8; MGC131757;
Gene ID 656
mRNA Refseq NM_001720
Protein Refseq NP_001711
MIM 602284
UniProt ID P34820
Chromosome Location 1p35-p32
Pathway Hedgehog signaling pathway, organism-specific biosystem; Hedgehog signaling pathway, conserved biosystem; TGF-beta signaling pathway, organism-specific biosystem; TGF-beta signaling pathway, conserved biosystem;
Function cytokine activity; growth factor activity;

Not For Human Consumption!

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