Recombinant Human BIRC6 protein, His-tagged

• Cat.No.: BIRC6-1170H
• Product Overview: Recombinant Human BIRC6 aa. (Arg4576~Leu4857 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. This protein inhibits apoptosis by facilitating the degradation of apoptotic proteins by ubiquitination.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Arg4576~Leu4857
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 5.4.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: BIRC6 baculoviral IAP repeat containing 6 [ Homo sapiens (human) ]
• Official Symbol: BIRC6
• Synonyms: BIRC6; baculoviral IAP repeat containing 6; BRUCE; APOLLON; baculoviral IAP repeat-containing protein 6; BIR repeat-containing ubiquitin-conjugating enzyme; RING-type E3 ubiquitin transferase BIRC6; ubiquitin-conjugating BIR-domain enzyme apollon
• Gene ID: 57448
• mRNA Refseq: NM_016252.3
• Protein Refseq: NP_057336.3
• UniProt ID: Q9NR09