Recombinant human BFIT, His-tagged

• Cat.No.: BFIT-2436H
• Product Overview: Recombinant human BFIT protein, fused to His-tag at N-terminus, was expressed in E.coli

Specification

• Description: BFIT is a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. The protein has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 29.9kDa (268aa)
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSNRTS RKSALRAGND SAMADGEGYR NPTEVQMSQL VLPCHTNQRG ELSVGQLLKWIDTTACLSAE RHAGCPCVTA SMDDIYFEHT ISVGQVVNIK AKVNRAFNSS MEVGIQVASE DLCSEKQWNV CKALATFVAR REITKVKLKQ ITPRTEEEKM EHSVAAERRR MRLVYADTIK DLLANCAIQG DLESRDCSRM VPAEKTRVES VELVLPPHAN HQGNTFGGQI MAWMENVA
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ACOT11?acyl-CoA thioesterase 11 [?Homo sapiens?(human) ]
• Official Symbol: ACOT11
• Synonyms: ACOT11; BFIT; THEA; THEM1; STARD14; acyl-CoA thioesterase 11; acyl-coenzyme A thioesterase 11; START domain containing 14; acyl-CoA thioester hydrolase 11; adipose-associated thioesterase; brown fat-inducible thioesterase; thioesterase, adipose associated; thioesterase superfamily member 1; StAR-related lipid transfer (START) domain containing 14; EC 3.1.2.-
• Gene ID: 26027
• mRNA Refseq: NM_015547
• Protein Refseq: NP_056362
• MIM: 606803
• UniProt ID: Q8WXI4
• Chromosome Location: 1p32.3
• Function: acyl-CoA hydrolase activity; carboxylesterase activity