Recombinant Human BCAT2, His-tagged

• Cat.No.: BCAT2-15863H
• Product Overview: Recombinant human BCAT2 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 28-392aa
• Description: BCAT2 belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. This protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. It may also function as a transporter of branched chain alpha-keto acids.
• Form: Liquid. 20mM Tris-HCl buffer (pH 7.5) containing 10% glycerol 0.2M NaCl, 1mM DTT
• Molecular Mass: 43.9 kDa(390aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASSSF KAADLQLEMT QKPHKKPGPG EPLVFGKTFT DHMLMVEWND KGWGQPRIQP FQNLTLHPAS SSLHYSLQLF EGMKAFKGKD QQVRLFRPWL NMDRMLRSAM RLCLPSFDKL ELLECIRRLI EVDKDWVPDA AGTSLYVRPV LIGNEPSLGV SQPTRALLFV ILCPVGAYFP GGSVTPVSLL ADPAFIRAWV GGVGNYKLGG NYGPTVLVQQ EALKRGCEQV LWLYGPDHQL TEVGTMNIFV YWTHEDGVLE LVTPPLNGVI LPGVVRQSLL DMAQTWGEFR VVERTITMKQ LLRALEEGRV REVFGSGTAC QVCPVHRILY KDRNLHIPTM ENGPELILRF QKELKEIQYG IRAHEWMFPV
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: BCAT2 branched chain amino-acid transaminase 2, mitochondrial [ Homo sapiens ]
• Official Symbol: BCAT2
• Synonyms: BCAT2; branched chain amino-acid transaminase 2, mitochondrial; BCT2, branched chain aminotransferase 2, mitochondrial; branched-chain-amino-acid aminotransferase, mitochondrial; BCAM; placental protein 18; branched chain aminotransferase 2, mitochondrial; BCT2; PP18; BCATM
• Gene ID: 587
• mRNA Refseq: NM_001190
• Protein Refseq: NP_001181
• MIM: 113530
• UniProt ID: O15382
• Chromosome Location: 19
• Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: L-isoleucine transaminase activity; L-leucine transaminase activity; L-valine transaminase activity; branched-chain-amino-acid transaminase activity; transferase activity