Recombinant Human Branched Chain Amino-Acid Transaminase 2, Mitochondrial, His-tagged

• Cat.No.: BCAT2-1962H
• Product Overview: Recombinant human BCAT2protein, fused to His-tag at N-terminus, was expressed in E.coli and purifiedby using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: BCAT2 belongs to theclass-IV pyridoxal-phosphate-dependent aminotransferase family. This proteinforms a dimer that catalyzes the first step in the production of the branchedchain amino acids leucine, isoleucine, and valine. It may also function as atransporter of branched chain alpha-keto acids.
• Form: Liquid. 20mMTris-HCl buffer (pH 7.5) containing 10% glycerol 0.2M NaCl, 1mM DTT.
• Molecular Weight: 43.9 kDa(390aa),confirmed by MALDI-TOF.
• Purity: > 90% by SDS -PAGE.
• Concentration: 0.5mg/ml (determinedby Bradford assay).
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMASSSF KAADLQLEMT QKPHKKPGPG EPLVFGKTFT DHMLMVEWND KGWGQPRIQPFQNLTLHPAS SSLHYSLQLF EGMKAFKGKD QQVRLFRPWL NMDRMLRSAM RLCLPSFDKL ELLECIRRLIEVDKDWVPDA AGTSLYVRPV LIGNEPSLGV SQPTRALLFV ILCPVGAYFP GGSVTPVSLL ADPAFIRAWVGGVGNYKLGG NYGPTVLVQQ EALKRGCEQV LWLYGPDHQL TEVGTMNIFVYWTHEDGVLE LVTPPLNGVILPGVVRQSLL DMAQTWGEFR VVERTITMKQ LLRALEEGRV REVFGSGTAC QVCPVHRILY KDRNLHIPTMENGPELILRF QKELKEIQYG IRAHEWMFPV
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: BCAT2

Gene Information

• Gene Name: BCAT2 branched chain amino-acidtransaminase 2, mitochondrial [ Homo sapiens ]
• Synonyms: BCAT2; branchedchain amino-acid transaminase 2, mitochondrial; BCAM; BCT2; PP18;BCATM; branched-chain-amino-acid aminotransferase, mitochondrial; placentalprotein 18; branched chain aminotransferase 2, mitochondrial
• Gene ID: 587
• mRNA Refseq: NM_001190
• Protein Refseq: NP_001181
• MIM: 113530
• UniProt ID: O15382
• Chromosome Location: 19
• pathway: Branched-chain amino acid catabolism;Leucine degradation; Metabolic pathways; Metabolism; Metabolism of aminoacids and derivatives; Pantothenate and CoA biosynthesis; Valine
• Function: L-isoleucinetransaminase activity; L-leucine transaminase activity; L-valine transaminaseactivity; branched-chain-amino-acid transaminase activity; transferaseactivity