Recombinant Human B2M protein, His-tagged

• Cat.No.: B2M-443H
• Product Overview: Recombinant Human B2M (P61769) aa. (Gln22~Met119) fused with His tag was produced in HEK293F cells.

Specification

• Description: This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
• Source: HEK293F
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 13.2kDa; Accurate Molecular Mass: 13kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gln22~Met119
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: B2M beta-2-microglobulin [ Homo sapiens (human) ]
• Official Symbol: B2M
• Synonyms: B2M; beta-2-microglobulin; IMD43; beta chain of MHC class I molecules; beta-2-microglobin
• Gene ID: 567
• mRNA Refseq: NM_004048.2
• Protein Refseq: NP_004039.1
• UniProt ID: P61769