Recombinant Human Aspartoacylase, His-tagged

• Cat.No.: ASPA-1920H
• Product Overview: Recombinant humanASPA protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: ASPA-1920H
• Description: ASPA,also known as aspartoacylase, is expressed in liver, lung and kidney tissue,as well as in skeletal muscle and in cerebral white matter. Existing as ahomodimer, Aspartoacylase functions to catalyze the deacetylation ofN-acetylaspartic acid to produce acetate and L-aspartate.
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol 1mM DTT, 0.1M NaCl, 0.1mMPMSF.
• Molecular Weight: 38.1 kDa(336aa), confirmed by MALDI-TOF
• Purity: > 90%by SDS-PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSMTSCHIA EEHIQKVAIF GGTHGNELTG VFLVKHWLEN GAEIQRTGLE VKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLP YEVRRAQEIN HLFGPKDSED SYDIIFDLHN TTSNMGCTLI LEDSRNNFLI QMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGI EVGPQPQGVL RADILDQMRK MIKHALDFIH HFNEGKEFPP CAIEVYKIIE KVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDG KTIPLGGDCT VYPVFVNEAA YYEKKEAFAK TTKLTLNAKS IRCCLH
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Alanineand aspartate metabolism; Alanine; Histidine metabolism

Gene Information

• Gene Name: ASPA aspartoacylase [ Homosapiens ]
• Official Symbol: ASPA
• Synonyms: ASPA; aspartoacylas; ASP; ACY2; ACY-2; aminoacylase2; aminoacylase-2; EC 3.5.1.15; spartoacylase (aminoacylase 2, Canavandisease); Canavan disease
• Gene ID: 443
• mRNA Refseq: NM_000049
• Protein Refseq: NP_000040
• MIM: 608034
• UniProt ID: P45381
• Chromosome Location: 17p13.3
• Function: aminoacylase activity; aspartoacylaseactivity; hydrolase activity; metal ion binding