Recombinant Human ARL5B, His-tagged

• Cat.No.: ARL5B-3614H
• Product Overview: Recombinant human ARL5B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: ADP-ribosylation factor-like protein 5B, also known as ARL5B, belongs to a family of proteins that are structurally similar to ADP-ribosylation factors. ARLs and ARFs are part of the RAS superfamily of regulatory GTPases. ARL5B is most closely related to ARL5, with which it shares 80% sequence identity. Human ARL5B shares 100% identity with mouse ARL8 and 71% identity with the Drosophila homolog. Two isoforms of ARL8 exist as a result of alternative splicing events.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT,30% glycerol, 0.1M NaCl, 1mM EDTA
• Molecular Weight: 22.5 kDa (199aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGLIFAKLWS LFCNQEHKVI IVGLDNAGKT TILYQFLMNE VVHTSPTIGS NVEEIVVKNT HFLMWDIGGQESLRSSWNTY YSNTEFIILV VDSIDRERLA ITKEELYRML AHEDLRKAAV LIFANKQDMK GCMTAAEISK YLTLSSIKDH PWHIQSCCAL TGEGLCQGLE WMTSRIGVR
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ARL5B ADP-ribosylation factor-like 5B [ Homo sapiens ]
• Official Symbol: ARL5B
• Synonyms: ARL5B; ARL8; ADP-ribosylation factor-like protein 5B;OTTHUMP00000019260;ADP-ribosylation factor-like 8;ADP-ribosylation-like factor 8;ADP-ribosylation factor-like protein 8
• Gene ID: 221079
• mRNA Refseq: NM_178815
• Protein Refseq: NP_848930
• MIM: 608909
• UniProt ID: Q96KC2
• Chromosome Location: 10p12.31
• Function: GTP binding; nucleotide binding