Recombinant Human APEX2 Protein, MYC/DDK-tagged

• Cat.No.: APEX2-2309H
• Product Overview: Recombinant human APEX2 protein, fused to MYC/DDK-tagged at C-terminus, was expressed in HEK293

Specification

• Description: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012].
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 57.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: APEX2 APEX nuclease (apurinic/apyrimidinic endonuclease) 2 [ Homo sapiens ]
• Official Symbol: APEX2
• Synonyms: APE2; APEXL2; XTH2; ZGRF2
• Gene ID: 27301
• mRNA Refseq: NM_014481
• Protein Refseq: NP_055296
• MIM: 300773
• UniProt ID: Q9UBZ4