Recombinant Human AP4B1 293 Cell Lysate

Cat.No. : AP4B1-8806HCL
  • Specification
  • Gene Information
  • Related Products
  • Download
Description : Antigen standard for adaptor-related protein complex 4, beta 1 subunit (AP4B1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name AP4B1 adaptor-related protein complex 4, beta 1 subunit [ Homo sapiens ]
Official Symbol AP4B1
Synonyms AP4B1; adaptor-related protein complex 4, beta 1 subunit; spastic paraplegia 47 , SPG47; AP-4 complex subunit beta-1; beta 4 subunit of AP 4; BETA 4; beta4-adaptin; spastic paraplegia 47; beta 4 subunit of AP-4; CPSQ5; SPG47; BETA-4;
Gene ID 10717
mRNA Refseq NM_001253852
Protein Refseq NP_001240781
MIM 607245
UniProt ID Q9Y6B7
Chromosome Location 1p13.2
Pathway Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Membrane Trafficking, organism-specific biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem;
Function binding; protein transporter activity; transporter activity;

Not For Human Consumption!

Inquiry

  • Reviews
  • Q&As

Customer Reviews (0)

Write a review

Q&As (0)

Ask a question

Ask a Question for All AP4B1 Products

Required fields are marked with *

My Review for All AP4B1 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon