Recombinant Human AHNAK protein, His & T7-tagged

• Cat.No.: AHNAK-1699H
• Product Overview: Recombinant Human AHNAK aa. (Met1~Asp300 (Accession # Q09666)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. A much shorter variant encoding a 17 kDa isoform exists for this gene, and the shorter isoform initiates a feedback loop that regulates alternative splicing of this gene.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met1~Asp300
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 5.3

Gene Information

• Gene Name: AHNAK AHNAK nucleoprotein [ Homo sapiens (human) ]
• Official Symbol: AHNAK
• Synonyms: AHNAK; AHNAK nucleoprotein; PM227; AHNAKRS; neuroblast differentiation-associated protein AHNAK; AHNAK-related; desmoyokin
• Gene ID: 79026
• mRNA Refseq: NM_001346445.1
• Protein Refseq: NP_001333374.1
• UniProt ID: Q09666