Recombinant Human AGT Protein, His-tagged

• Cat.No.: AGT-95H
• Product Overview: Recombinant Human AGT Protein (Asp34-Ala485) with N-His tag was expressed in 293F.

Specification

• Description: The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure, body fluid and electrolyte homeostasis, and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
• Source: HEK293F
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 51.4 kDa
  Accurate Molecular Mass: 50 kDa
• Protein length: Asp34-Ala485
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: AGT angiotensinogen (serpin peptidase inhibitor, clade A, member 8) [ Homo sapiens (human) ]
• Official Symbol: AGT
• Synonyms: AGT; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); SERPINA8; angiotensinogen; alpha 1 antiproteinase; antitrypsin; serpin A8; angiotensin I; angiotensin II; pre-angiotensinogen; alpha-1 antiproteinase, antitrypsin; serine (or cysteine) proteinase inhibitor; ANHU
• Gene ID: 183
• mRNA Refseq: NM_000029
• Protein Refseq: NP_000020
• MIM: 106150
• UniProt ID: P01019