Recombinant Human Angiotensinogen, His&GST-tagged

• Cat.No.: AGT-2183H
• Product Overview: Recombinant Human Angiotensinogen(Arg35~Ala485), fused with two N-terminal Tags, His-tag and GST-tag, was expressed in Prokaryotic expression.

Specification

• Description: The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
• Source: E.coli
• Species: Human
• Tag: His&GST
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 76.7kDa
• AA Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFGPLGSEF-RVYIHP FHLVIHNEST CEQLAKANAGKPKDPTFIPA PIQAKTSPVD EKALQDQLVL VAAKLDTEDK LRAAMVGMLA NFLGFRIYGMHSELWGVVHG ATVLSPTAVF GTLASLYLGA LDHTADRLQA ILGVPWKDKN CTSRLDAHKVLSALQAVQGL LVAQGRADSQ AQLLLSTVVG VFTAPGLHLK QPFVQGLALY TPVVLPRSLDFTELDVAAEK IDRFMQAVTG WKTGCSLMGA SVDSTLAFNT YVHFQGKMKG FSLLAEPQEFWVDNSTSVSV PMLSGMGTFQ HWSDIQDNFS VTQVPFTESA CLLLIQPHYA SDLDKVEGLTFQQNSLNWMK KLSPRTIHLT MPQLVLQGSY DLQDLLAQAE LPAILHTELN LQKLSNDRIRVGEVLNSIFF ELEADEREPT ESTQQLNKPE VLEVTLNRPF LFAVYDQSAT ALHFLGRVANPLSTA
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Protein length: 35-485 a.a.

Gene Information

• Gene Name: AGT angiotensinogen (serpin peptidase inhibitor, clade A, member 8) [ Homo sapiens (human) ]
• Official Symbol: AGT
• Synonyms: AGT; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); ANHU; FLJ92595; FLJ97926; SERPINA8; angiotensinogen; serpin A8; angiotensin I; angiotensin II; pre-angiotensinogen; alpha-1 antiproteinase, antitrypsin; serine (or cysteine) proteinase inhibitor; Angiotensin-1; Ang I; Angiotensin-2; Ang II; Angiotensin-3; Ang III; Angiotensin III
• Gene ID: 183
• mRNA Refseq: NM_000029
• Protein Refseq: NP_000020
• MIM: 106150
• UniProt ID: P01019
• Chromosome Location: 1q42.2
• Pathway: Hypertrophic cardiomyopathy (HCM); Renin-angiotensin system; Vascular smooth muscle contraction
• Function: acetyltransferase activator activity; growth factor activity; hormone activity; serine-type endopeptidase inhibitor activity; type 1 angiotensin receptor binding; type 2 angiotensin receptor binding