Recombinant Human AGC, His-tagged, S tagged

• Cat.No.: AGC-3238H
• Product Overview: Aggrecan (AGC)

Specification

• Description: This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
• Source: E. Coli
• Species: Human
• Tag: His&S
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 24.2kDa
• Protein length: 2415
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of AGC recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: ACAN aggrecan [ Homo sapiens ]
• Official Symbol: ACAN
• Synonyms: AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1
• Gene ID: 176
• mRNA Refseq: NM_001135
• Protein Refseq: NP_001126
• MIM: 155760
• UniProt ID: P16112
• Chromosome Location: 15q26.1
• Pathway: Degradation of the extracellular matrix, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem
• Function: carbohydrate binding; extracellular matrix structural constituent