Active Recombinant Human ACAN Protein, His-tagged

• Cat.No.: ACAN-671H
• Product Overview: Recombinant Human ACAN (Accession # NP_037359) Val20-Gly675, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Val20
• Form: Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its binding ability in a functional ELISA.Biotinylated hyaluronan immobilized on an EvenCoatTM Streptavidin Microplate at 0.1 µg/mL (100 µL/well) can bind rhAggrecan with a linear range of 8 - 500 ng/mL.Can also be used as a protein substrate for proteases such as MMPs and ADAMTSs. The cleavage products can be analyzed by either SDS-PAGE or western blot using anti-human aggrecan antibodies.
• Molecular Mass: Recombinant Human ACAN, His-tagged has a calculated MW of 74 kDa. In SDS-PAGE migrates as 120 kDa, reducing conditions.
• Protein length: Val20-Gly675
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ACAN aggrecan [ Homo sapiens ]
• Official Symbol: ACAN
• Synonyms: ACAN; aggrecan; AGC1, aggrecan 1 , chondroitin sulfate proteoglycan 1 , CSPG1, MSK16; aggrecan core protein; aggrecan proteoglycan; CSPGCP; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1; AGC1; SEDK; AGCAN; CSPG1; MSK16
• Gene ID: 176
• mRNA Refseq: NM_001135
• Protein Refseq: NP_001126
• MIM: 155760
• UniProt ID: P16112
• Chromosome Location: 15q26.1