Recombinant Human ADP-Ribosylation Factor-Like 14, His-tagged

• Cat.No.: ARL14-2489H
• Product Overview: Recombinant human ARL14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 23.8 kDa.

Specification

• Description: ARL14 is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein family of Ras-related GTPases. MHC class II molecules present peptides to T helper cells to facilitate immune responses and are strongly linked to autoimmune diseases. MHC-II transport was controlled by the GTPase ARL14/ARF7, which recruits the motor myosin 1E via an effector protein ARF7EP.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl, 1mM EDTA.
• Molecular Mass: 23.8kDa (212aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher).
• Protein length: 1-192aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSLGSKNPQ TKQAQVLLLG LDSAGKSTLL YKLKLAKDIT TIPTIGFNVE MIELERNLSL TVWDVGGQEK MRTVWGCYCE NTDGLVYVVD STDKQRLEES QRQFEHILKN EHIKNVPVVL LANKQDMPGA LTAEDITRMF KVKKLCSDRN WYVQPCCALT GEGLAQGFRK LTGFVKSHMK SRGDTLAFFK QN
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ARL14 ADP-ribosylation factor-like 14 [ Homo sapiens ]
• Official Symbol: ARL14
• Synonyms: ARL14; ADP-ribosylation factor-like 14; ADP ribosylation factor 7 , ARF7; ADP-ribosylation factor-like protein 14; FLJ22595; ADP-ribosylation factor 7; ARF7
• Gene ID: 80117
• mRNA Refseq: NM_025047
• Protein Refseq: NP_079323
• MIM: 614439
• UniProt ID: Q8N4G2
• Chromosome Location: 3q25.33
• Function: GTP binding; nucleotide binding; protein binding