Recombinant Human ADD1 293 Cell Lysate

• Cat.No.: ADD1-9017HCL

Specification

• Description: Antigen standard for adducin 1 (alpha) (ADD1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: ADD1 adducin 1 (alpha) [ Homo sapiens ]
• Official Symbol: ADD1
• Synonyms: ADD1; adducin 1 (alpha); alpha-adducin; erythrocyte adducin alpha subunit; ADDA; MGC3339; MGC44427
• Gene ID: 118
• mRNA Refseq: NM_014189
• Protein Refseq: NP_054908
• MIM: 102680
• UniProt ID: P35611
• Chromosome Location: 4p16.3
• Pathway: Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Caspase-mediated cleavage of cytoskeletal proteins, organism-specific biosystem; Diabetes pathways, organism-specific biosystem
• Function: actin binding; actin filament binding; calmodulin binding; metal ion binding; protein heterodimerization activity; protein homodimerization activity; spectrin binding; transcription factor binding