Recombinant Human 3-hydroxyanthranilate 3,4-dioxygenase, His-tagged

• Cat.No.: HAAO-2403H
• Product Overview: Recombinant humanHAAO protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques

Specification

• Cat. No.: HAAO-2403H
• Description: HAAO is a monomericcytosolic protein belonging to the family of intramolecular dioxygenasescontaining nonheme ferrous iron. This protein catalyzes the synthesis ofquinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxinwhose toxicity is mediated by its ability to activate glutamateN-methyl-D-aspartate receptors. Increased cerebral levels of QUIN mayparticipate in the pathogenesis of neurologic and inflammatory disorders.HAAO has been suggested to play a role in disorders associated with alteredtissue levels of QUIN
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Weight: 35kDa (310aa),confirmed by MALDI-TOF
• Purity: > 90% by SDS -PAGE
• Concentration: 0.5mg/ml (determinedby BCA assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMERRLG VRAWVKENRG SFQPPVCNKL MHQEQLKVMF IGGPNTRKDY HIEEGEEVFYQLEGDMVLRV LEQGKHRDVV IRQGEIFLLP ARVPHSPQRF ANTVGLVVER RRLETELDGL RYYVGDTMDVLFEKWFYCKD LGTQLAPIIQ EFFSSEQYRT GKPIPDQLLK EPPFPLSTRS IMEPMSLDAW LDSHHRELQAGTPLSLFGDT YETQVIAYGQ GSSEGLRQNV DVWLWQLEGS SVVTMGGRRL SLAPDDSLLV LAGTSYAWERTQGSVALSVT QDPACKKPLG
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°Cor -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: HAAO3-hydroxyanthranilate 3,4-dioxygenase [ Homo sapiens ]
• Official Symbol: HAAO
• Synonyms: HAAO; 3-hydroxyanthranilate3,4-dioxygenase; 3 HAO; 3 hydroxyanthranilate 3 4 dioxygenase; 3hydroxyanthranilate dioxygenase; 3 hydroxyanthranilate oxygenase; 3hydroxyanthranilic acid dioxygenase; 3-HAO; 3-hydroxyanthranilate 3;3-hydroxyanthranilate oxygenase; 3-hydroxyanthranilic acid dioxygenase;3HAO_HUMAN; 4-dioxygenase; haao; HAD; HAO; HAD; 3-hydroxyanthranilateoxygenase; 3-hydroxyanthranilic acid dioxygenase; HAO; 3-HAO
• Gene ID: 23498
• mRNA Refseq: NM_012205
• Protein Refseq: NP_036337
• MIM: 604521
• Uniprot ID: P46952
• Chromosome Location: 2p
• Pathway: Metabolic pathways,organism-specific biosystem; Metabolism, organism-specific biosystem;Metabolism of amino acids and derivatives, organism-specific biosystem; NADbiosynthesis II (from tryptophan), conserved biosystem; Tryptophancatabolism, organism-specific biosystem
• Function: 3-hydroxyanthranilate3,4-dioxygenase activity; 3-hydroxyanthranilate 3,4-dioxygenase activity;electron carrier activity; ferrous iron binding; metal ion binding