Recombinant Hepatitis B virus HBsAg L-protein genotype C Protein

• Cat.No.: HBsAg L-14V
• Product Overview: Recombinant Hepatitis B virus HBsAg L-protein genotype C Protein was expressed in Saccharomyces cerevisiae. Particle (L-protein inserted in lipid bilayer) with mean particle size of about 70nm by dynamic light scattering method (about 20 nm by electronic microscope)

Specification

• Species: Hepatitis B virus
• Source: S.Cerevisiae
• Description: Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. Most of commercially available HBsAg is composed of either S-protein alone or a mixture of Sand M-proteins. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection.
  The present product, HBsAg, L-protein (L antigen) is a particulate antigen composed of L protein, and can be used as a tool to investigate the mechanism of HBV infection, ELISA and others. Four major genotypes of products are available.
• Form: Lyophilized white powder.
• Purity: over 95%
• Usage: HBV mimetics, Standard antigen for ELISA, western, and others.
• Storage: 20 centigrade(3 years guarantee after dispatch).
• Reconstitution: For 100 µg vial, added 500 µL of water to the vial that makes a antigen solution at 0.2 mg/mL in PBS (137 mM NaCl, 8.1 mM Na2HPO4・12H2O, 2.68 mM KCl, 1.47 mM KH2PO4, pH 7.2 - 7.4) containing 1% sucrose.
• Warning: L protein may be adsorbed on plastic tubes especially low concentration (below 100ng/mL). Please use protein low bind tubes at lower concentration.

Gene Information

• Gene Name: NEWENTRY Record to support submission of GeneRIFs for a gene not in Gene (Human hepatitis B virus; hepatitis B virus (HBV); hepatitis B virus HBV; hepatitis B virus, HBV; human hepatitis B virus HBV). [ Hepatitis B virus ]
• Official Symbol: NEWENTRY
• Synonyms: NEWENTRY
• Gene ID: 2828293

Q&As

What size is the recombinant HBsAg? Is it the L, M or S version of the antigen?

The HBsAg proteins are generally provided in 100ug, 500ug, and 1mg packing sizes. We can also aliquot based on your specific requirements. Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. As the sequence of the below antigens is confidential, we cannot tell that if they are L, M, or S versions.