Recombinant Hepatitis B virus HBsAg M Protein

Cat.No. : HBsAg M-15V
Product Overview : Recombinant Hepatitis B virus HBsAg M Protein was expressed in Saccharomyces cerevisiae. Particle (M-protein inserted in lipid bilayer) with mean particle size of about 65 nm by dynamic light scattering method (about 20 nm by electronic microscope).
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Description : Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. Most of commercially available HBsAg is composed of either S-protein alone or a mixture of Sand M-proteins. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection.
The present product, HBsAg, M-protein (M antigen) is a particulate antigen composed of M protein, and can be used as a tool to investigate the mechanism of HBV infection, ELISA and others. The product is genotypes C.
Source : Saccharomyces cerevisiae
Species : Hepatitis B virus
Form : Lyophilized white powder.
Purity : over 95%
Usage : Standard antigen for ELISA, western, and others
Storage : 20 centigrade(3 years guarantee after dispatch).
Reconstitution : For 100 µg vial, added 500 µL of water to the vial that makes a antigen solution at 0.2 mg/mL in PBS (137 mM NaCl, 8.1 mM Na2HPO4・12H2O, 2.68 mM KCl, 1.47 mM KH2PO4, pH 7.2 - 7.4) containing 1% sucrose.
Warning : M protein may be adsorbed on plastic tubes especially low concentration (below 100ng/mL). Please use protein low bind tubes at lower concentration.

Not For Human Consumption!

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