Recombinant Full Length Human UNG Protein, C-Flag-tagged

• Cat.No.: UNG-1019HFL
• Product Overview: Recombinant Full Length Human UNG Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 34.5 kDa
• AA Sequence: MIGQKTLYSFFSPSPARKRHAPSPE PAVQGTGVAGVPEESGDAAAIPAKK APAGQEEPGTPPSSPLSAEQ LDRIQRNKAAALLRLAARNVPVGFG ESWKKHLSGEFGKPYFIKLMGFVAE ERKHYTVYPPPHQVFTWTQM CDIKDVKVVILGQDPYHGPNQAHGL CFSVQRPVPPPPSLENIYKELSTDI EDFVHPGHGDLSGWAKQGVL LLNAVLTVRAHQANSHKERGWEQFT DAVVSWLNQNSNGLVFLLWGSYAQK KGSAIDRKRHHVLQTAHPSP
  LSVYRGFFGCRHFSKTNELLQKSGK KPIDWKELTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Primary immunodeficiency

Gene Information

• Gene Name: UNG uracil DNA glycosylase [ Homo sapiens (human) ]
• Official Symbol: UNG
• Synonyms: DGU; UDG; UNG1; UNG2; HIGM4; HIGM5; UNG15
• Gene ID: 7374
• mRNA Refseq: NM_080911.3
• Protein Refseq: NP_550433.1
• MIM: 191525
• UniProt ID: P13051

Reviews

09/22/2022

The technician guided me through the process, which led to satisfactory results.

07/14/2022

The UNG protein product received was as advertised, top quality and good value for money.

05/04/2022

Prompt delivery helped me get satisfactory experimental results in a short period of time.

Q&As

What biological processes are UNG involved in?

UNG is involved in DNA repair, base excision repair and apoptosis.

Where is UNG located?

UNG is localized in the nucleus, nucleoplasm, and mitochondria.

What protein does UNG shear to produce?

UNG can be sheared to produce UNG1 and UNG2.

Where is UNG primarily expressed?

UNG is mainly expressed in testis, thymus and skeletal muscle.