Recombinant Full Length Human Protein Kinase C, Zeta, GST-tagged, Active

• Cat.No.: PRKCZ-409H
• Product Overview: Recombinant full-length human PRKCZ was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=93kDa.

Specification

• Cat. No.: PRKCZ-409H
• Description: PKCζ is an atypical isoform of the PKC family. PKCζ is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Overexpression of PKCζ and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation. Overexpression of PKCζ also potentiates phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. PKCζ is an upstream modulator of p70S6K, an important regulator of cell proliferation.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Protein length: 1-592 a.a.

Gene Information

• Gene Name: PRKCZ protein kinase C, zeta [ Homo sapiens ]
• Synonyms: PRKCZ; protein kinase C, zeta; PKC2; PKC-ZETA; EC 2.7.11.13; nPKC-zeta; Protein kinase C zeta type
• Gene ID: 5590
• mRNA Refseq: NM_001033581
• Protein Refseq: NP_001028753
• MIM: 176982
• UniProt ID: Q05513
• Chromosome Location: 1p36.33-p36.2
• Pathway: Chemokine signaling pathway; Endocytosis; Insulin signaling pathway; Tight junction; Type II diabetes mellitus
• Function: ATP binding; insulin receptor substrate binding; metal ion binding; nucleotide binding; phospholipase binding; potassium channel regulator activity; protein kinase C activity; transferase activity; zinc ion binding